Two other studies verified the hypoglycemic and antidepressant effects of Glucose-Lowering and Relieving Depression Decoction for Tonifying the Kidney-yin (Zuogui Jiangtang Jieyu prescription, ZGJTJY) (Wang et al., 2014; Yang et al., 2020), showing that ZGJTJY can effectively regulate the insulin signaling pathway and improve insulin resistance in the hippocampus of rats with diabetes-related depression (Yang et al., 2020). The gene discussed is INS; the disease is major depressive disorder.