Treatment of NSCLC cell lines H1666 and CAL-12-T cells (Class 3, BRAFG466V; heterozygous and homozygous, respectively) with SJF-0628 caused a dose-dependent loss in BRAF protein levels in both cell lines (CAL-12T: DC50 = 23 nM, DMAX > 90%) (H1666 cells: DC50 = 29 nM, DMAX > 80%) (Fig. 1g, Supplementary Fig. 3d) as well as substantial p-ERK inhibition but showed slight stabilization at SJF-0628 concentrations higher than 1 μM. This evidence concerns the gene BRAF and non-small cell lung carcinoma.