Strikingly, the enhancement of RhoA-GTP (Fig. 4d), cell migration, and invasion in 143B cells in vitro (Fig. 4e), as well as lung metastases of 143B-Luc cells in the orthotopic osteosarcoma metastasis model in vivo (Fig. 4f–h and Supplementary Fig. 4g) were observed by either Rab22a-NeoF1-exo or Rab22a-NeoF1-shPYK2#NC-exo, but not by either Rab22a-NeoF1-shPYK2#1-exo or Rab22a-NeoF1-shPYK2#2-exo compared to Vector-exo. The gene discussed is RAB22A; the disease is osteosarcoma.