Furthermore, using the orthotopic osteosarcoma metastasis model in vivo and the tail vein injection metastasis model in vivo, the lung metastases of 143B-Luc cells were significantly increased in mice pre-educated by Rab22a-NeoF1-CM compared to those pre-educated by Vector-CM (Fig. 5e–g and Supplementary Fig. 5a). Here, RAB22A is linked to osteosarcoma.