To address whether NO and cytokine production was due to phagocytosis or to contact with gametocytes, experiments were repeated in the presence of cytochalasin D. Results show that cytochalasin D did not modify the production of NO and increased the production of both TNF and MIP-2 induced by late GCT (Fig. 5) proving that phagocytosis was not involved in cytokines and NO induction. The gene discussed is TNF; the disease is granular cell tumor.