Total calpain, atrogin-1, MuRF-1, LC3-II, and Beclin-1 levels were higher in mice with endotoxemia subjected to VT = 10 mL/kg than in mice in the other MV treatment groups and the non-ventilated control mice (Figure 2E and Figure 3). This evidence concerns the gene BECN1 and serum lipopolysaccharide activity.