A black pixel analysis derivative adapted for single tumor mouse models (Method S6 and Figures S11) was used to quantify the accumulation of the PSMA-targeted MNPs in PSMA(+) tumors of Group 2 mice compared to those of Group 3 mice, 18 h, 42 h, and 66 h post-PDT. Here, FOLH1 is linked to neoplasm.