The results of the Annexin V/PI staining assay indicated that E35 induced the apoptosis of MM cells (Figure 2) and downregulated mRNA/protein expression of apoptosis-related genes, including C-Myc, Bcl-2, Mcl-1, and Pim2 (Figures 3 and 4). Here, PIM2 is linked to Miyoshi myopathy.