To test the reactivity of the CAR-T cells against their intended TAM targets, CD11b+ cells (containing TAMs) were isolated from the ascites of ID8 tumor-bearing Folr2 (gene encoding for FRβ) wild-type (WT) (Folr2 wt) or Folr2 knockout (KO) (Folr2−/−) mice and cocultured with mFRβ CAR, control hCD19 CAR, or untransduced (UTD) T cells. This evidence concerns the gene FOLR2 and neoplasm.