In thisresearch, the signaling pathways of two widely used therapeutic approachesin colon cancer, EGFR and VEGFR inhibition, and the effectivenessof immune checkpoint inhibition are estimated in advance to facilitatetherapeutic decision making by examining microsatellite instability,and the loss of expression of the mismatch repair proteins responsiblefor it was examined by analyzing the changes in the gene expressionprofile of the proteins associated with the appropriate Gene Ontologyterms. This evidence concerns the gene EGFR and malignant colon neoplasm.