In the current study, the adeno-associated virus-mediated IL-7 or lentivirus-mediated transcription factors Id3, Bcl6, Bach2, and Blimp1 were immunized, together with the TB fusion protein-based vaccine Mtb10.4-HspX (MH) [27] plus ESAT6-Ag85B-MPT64(190–198)-Mtb8.4-Rv2626c (LT70) [28] to investigate whether they can cause the TB subunit vaccine to induce long-term T cell-mediated and antibody-mediated immune responses. The gene discussed is ID3; the disease is tuberculosis.