HMOX1 and myocardial infarction: mRNA and protein expression of Keap1, nuclear Nrf2, and HO-1 were significantly elevated, while cytosolic Nrf2 protein expression was non-significantly reduced in the myocardial tissues of ISO-induced MI animals, indicating that ISO caused Nrf2 activation due to the presence of ROS, which by itself has the ability to activate Nrf2.