mRNA and protein expression of Keap1, nuclear Nrf2, and HO-1 were significantly elevated, while cytosolic Nrf2 protein expression was non-significantly reduced in the myocardial tissues of ISO-induced MI animals, indicating that ISO caused Nrf2 activation due to the presence of ROS, which by itself has the ability to activate Nrf2. This evidence concerns the gene NFE2L2 and myocardial infarction.