After exclusion of PCOS women with regular menses (n = 19), VD effects on FSH levels (mean treatment effect 0.271, 95% CI 0.27 to 2.06, p = 0.011) and LH/FSH ratio (mean treatment effect −0.401, 95% CI −0.705 to −0.097, p = 0.010) remained stable. This evidence concerns the gene PLOD1 and polycystic ovary syndrome.