Our results show that isolated cells from seminiferous tubules of immature mice ten (10) days post CP treatment still have spermatogenic cells that were positively stained for pre-meiotic (VASA, CD9, GFR-α, α-6-INTEFRIN, and c-KIT), meiotic (BOULE and CREM), and to meiotic/post-meiotic (ACROSIN) markers [before culture (BC)] as examined by immunofluorescence staining using specific antibodies for each marker (Figure 2A,B) and summarized in Table 1. This evidence concerns the gene DDX4 and breast cancer.