In our study, we found a significant decrease in the number of Brn3a+ RGCs (total value) after 120 days of the disease, which coincided with the damage observed in the RGCs in other ALS models [22,27,28], as well as with the thinning of the NFL observed by OCT in patients with ALS compared to the controls [21,22,24,25,26]. The gene discussed is POU4F1; the disease is amyotrophic lateral sclerosis.