Data obtained using 401 blood samples from 208 HCC patients and 193 liver cirrhosis patients showed that sensitivity and specificity of the tests for AFP were 62% and 90.2%, for PIVKA-II were 51% and 91.2%, for OPN 46.2% and 80.3%, and for DKK1 50% and 80.8%, respectively [161]. This evidence concerns the gene SPP1 and hepatocellular carcinoma.