To test this hypothesis, we independently overexpressed N-terminally His6-tagged Bag-1S (His6Bag-1S), Bag-1L (His6Bag-1L), and Beclin 1 (His6Beclin 1) proteins in MDA-MB-231, MCF-7, and BT-474 human breast cancer cell lines since they exhibit differences in their prognosis to the disease progression due to their origin and the rates of survival and recurrence. The gene discussed is BECN1; the disease is breast cancer.