With the ability of PC7A to activate STING and cytosolic delivery of cGAMP, we demonstrate that cGAMP–PC7A NPs achieved rapid and sustained STING activation across 6–48 h in both MC38 tumours and draining lymph nodes (Supplementary Fig. 9), which enable an optimal time window for DC maturation and T-cell priming (normally requires 1–2 d)52,53. The gene discussed is STING1; the disease is neoplasm.