Two-way ANOVA analysis showed a significant interaction effect between genotype and AAV in cumulative weight gain (F(1,27) = 4.262, p = 0.049), food intake (F(1,28) = 7.684, p = 0.001), and AUC (F(1,22) = 4.466, p = 0.046) substantiating the critical role of olfactory CB1 in overfeeding mediated obesity. The gene discussed is CNR1; the disease is obesity due to melanocortin 4 receptor deficiency.