As shown in Supplement file 2A,B, the results indicated that SERPINE1, CTLA4, F5, AGT, GAD1, CYP19A1, BBC3, and NOX4 were significantly overexpressed in GC samples, while the expression patterns of HBB, KIT, and GPX3 were decreased compared to those in normal tissues. The gene discussed is F5; the disease is gastric cancer.