For instance, breaking the positive feedback loop upon Piezo1 deletion leads to a decreased Ca2+ influx and ROS production, resulting in a combination of reduced SR Ca2+ content (Fig. 2f, g) and unsensitized SR Ca2+ release (Fig. 4a–c), which is expected to cause comprised heart pump function and the development of cardiomyopathy of the Piezo1-KO mice (Fig. 6). This evidence concerns the gene PIEZO1 and cardiomyopathy.