CBS and cancer: Besides CBS, AOAA has also been identified to inhibit some other enzymes including CSE and glutamic-oxaloacetic transaminase 1 (GOT1), which also play important roles in the regulation of cancer cell bioenergy.28 To further confirm the specific inhibitory effect of AOAA in our study, we applied AOAA to the CBS silenced cells and found AOAA significantly lost its anti-proliferative and pro-apoptotic effects in K562 cells.