These signaling pathways promote the cell survival and cell adhesion of hematopoietic stem cells, leading to form CML abnormal clones.5,34,35 To determine whether inhibition of CBS affects BCR-ABL expression or its phosphorylation, and the downstream PI3K/AKT, MAPK, ERK signaling pathways, Western blot analysis was performed to detect the phosphorylation levels of related proteins in the cells treated with different concentrations of AOAA or transfected with shRNA. The gene discussed is AKT1; the disease is chronic myelogenous leukemia, BCR-ABL1 positive.