In colon cancer, the inhibition of CBS can reduce the biological energy metabolism of tumor cells by reducing the production of endogenous H2S, thereby achieving an antitumor effect.18 By using sequence-specific shRNA to silence the expression of CBS or AOAA to inhibit its activity, we found that the inhibition of CBS not only reduced K562 cell proliferation, but also had pro-apoptotic and anti-migration effects in both cultured K562 cells and tumor xenografts. This evidence concerns the gene CBS and malignant colon neoplasm.