In our study, we mainly use adeno-associated virus (AAV)-shGITRL and LV-shGITRL to knockdown the expression of GITRL on the surface of DCs in vivo and in vitro, and then detect the differentiation of CD4+ T cells and its effect on the asthma phenotype, which provides a basis for immunotherapy of asthma and has important clinical significance. This evidence concerns the gene CD4 and asthma.