Previous study reported that the expression of the transcription factor helios has proven to be a useful marker for the identification of stable nTregs, while nTregs could induce the suppression of autoimmune disease.23 To further investigate the exact Treg cells subset, we performed the immunofluorescence staining of foxp3 and helios, then we found that foxp3 and helios double positive cells decreased in the B. adolescentis group with DSS treatment when compared to the PBS group (Figure 5e). Here, FOXP3 is linked to autoimmune disease.