TNF and colitis: A further increase in the expression of TNF-α and IL-6 was observed in mice fed a HFD with TNBS-induced colitis; however, when the SD and HFD mice were subjected to voluntary training, a significant decrease in the expression of TNF-α and IL-6 was observed when compared to respective values obtained in comparison with the sedentary TNBS group fed SD and HFD (p < 0.05) (Figure 5, upper and lower panels, respectively).