Furthermore, the trends of cyclin D1 and PD-L1 protein abundance in RCC-NC/miR-224-5p cells were similar to that in miRNA mimic-transfected RCC cells (Figure S6A), which could be reversed by miR-224-5p inhibitors (Figure S6B), or the transient overexpression of CCND1 (Figure 7B). Here, CCND1 is linked to renal cell carcinoma.