ALB and liver disorder: We adopted centrifugal ultrafiltration dialysis to obtain the data enabling us to determine binding constants for DBP and albumin and the free fraction for both 25OHD and 1,25(OH)2D shown in Table 1.(42, 43, 44, 45) Moreover, these studies enabled us to demonstrate the changes in the free fraction associated with changes in DBP levels during liver disease and pregnancy, data indicating the important contribution that measurement of the free hormone level could make in assessing hormone status in conditions in which DBP levels varied.