By comparing the effect observed in the ENS when using MOs, to the effect observed when using CRISPR-Cas9, four rare exonic de novo variants affecting the genes: DENN Domain Containing 3 (dennd3), Nucleolin (ncln), Nucleoporin 98 (nup98), and Thymus, Brain And Testes Associated (tbata), were identified to cause HSCR (Gui et al., 2017). The gene discussed is DENND3; the disease is Hirschsprung disease.