The three patients with virus negative OSCC containing p16 overexpression all had recurrent disease within 6 months after surgical resection [a T4N1M0 floor of mouth tumor, after an irradical resection the patient wished no further treatment (DFS = 0 months), OS = 27.4 months; a T4N0M0 oral cavity tumor (not otherwise specified), DFS = 5.4 months after resection, OS = 8.2 months; a T2N1M0 retromolar triangle tumor, DFS 4.8 months after resection, OS = 11.8 months]. The gene discussed is CDKN2A; the disease is oral cavity neoplasm.