To further validate the pro-EMT effect of IKBIP in glioma, we selected a series of EMT-related signaling pathways and biomarkers, which were then analyzed to determine their interaction with IKBIP and found that IKBIP showed robust correlation with PI3K/AKT-, hypoxia- and TGF-β-signaling pathway, suggesting that IKBIP might promote EMT process through these pathways. The gene discussed is AKT1; the disease is central nervous system cancer.