We also explored the possible signaling pathways and target genes of PTTG1 in ESCC by combining the target genes of PTTG1 (displayed by ChIP-seq), differentially expressed genes (DEGs) of ESCC, and PTTG1-related genes (Supplementary Figure 1), revealing the potential molecular mechanism of PTTG1 in ESCC and providing a new target for ESCC research. Here, PTTG1 is linked to esophageal squamous cell carcinoma.