Based on the existing research on PTTG1 in ESCC, this study used clinical samples to make tissue chips, performed immunohistochemistry (IHC) to detect the expression level of PTTG1 protein in ESCC tissues, and used our clinical sample tissues to conduct RNA sequencing (RNA-seq) to verify the expression of PTTG1 mRNA. Here, PTTG1 is linked to esophageal squamous cell carcinoma.