By using ischemia-reperfusion injury (IRI) as a model of experimental AKI, we found PD-L1 induced in early injury at day 3 and 7, whereas renal recovery at day 10 after ischemia resulted in normalization of PD-L1 expression (Figures 1A, B and Supplementary Figure 1A). This evidence concerns the gene CD274 and ischemia reperfusion injury.