However, the question remains to be answered is how the Nlrp3 inflammasome activation induced by Stx2 further causes HUS, by increasing the renal sensitivity to the Stx2 subunit A via upregulating the expression of Gb3 receptor on renal cell membrane, or by excessive inflammatory response via recruiting leukocytes to the renal local tissue. This evidence concerns the gene STX2 and hemolytic-uremic syndrome.