Despite previous research claiming the antiproliferative and pro-apoptotic effects on several cancer cell lines through the activation of caspase-dependent pathway, inhibition of Wnt/β-catenin signaling pathway and induction of a p53/MDM2-dependent mechanism, the study revealed a weak influence of tubeimoside-1 on the cellular viability and proliferation of breast cancer cells. This evidence concerns the gene TP53 and breast cancer.