GPT and steatosis: The db/db mice exhibited hyperglycaemia and features of MAFLD including a pale and enlarged liver, increased liver to body weight ratio, elevated serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT), raised serum triglyceride (TG) and total cholesterol (TC), lipid accumulation around the perisinusoidal areas in the form of macrovascular steatosis (Figure 1).