Different cellular models have been used to express wild-type MLC1 and GlialCAM or containing MLC mutations, such as Xenopus oocytes (Teijido et al., 2004), Spodoptera frugiperda (Sf9) cells (Ridder et al., 2011) and other cell lines such as fibroblast HeLa (López-Hernández et al., 2011b), COS-1 (Hwang et al., 2019), HEK293 or glioblastoma U373 (Ambrosini et al., 2008) and human U251 astrocytoma (Lanciotti et al., 2020). Here, MLC1 is linked to glioblastoma.