Previous semi-automated systems, such as Sholl concentric circle analysis used to identify cellular processes, ramification, and length of post-stroke GFAP+ astrocytes, did provide additional morphology-specific information, but lacked informative measures of ellipticity and other measures often associated with ischemia-induced immune cell alterations (Mestriner et al., 2015). The gene discussed is GFAP; the disease is Stroke.