In previous study, over expression of 9 selected gene biomarkers (ANLN, CCNA2, CDCA5, CKAP2L, DLGAP5, KIF4A, KPNA2, SHCBP1 and TPX2) can indicate poor prognosis in different cancer types, including lung cancer, colon cancer, breast cancer and bladder cancer [22–31]. The gene discussed is ANLN; the disease is malignant colon neoplasm.