To explore the effects of the PRR on YAP activation and observe whether the PRR mediates YAP pathway involvement in the pathogenesis of DCM, recombinant-adenoviruses-carried-PRR-gene (Ad-PRR), recombinant-adenoviruses-carried-PRR-shRNA (Ad-PRR-shRNA) and lentivirus-carried-YAP-shRNA (LV-YAP-shRNA) were constructed. Here, ATP6AP2 is linked to familial dilated cardiomyopathy.