miR-501-3p and WDR82 expression in cells were assessed as well and we found that versus the HBE cells, miR-501-3p was upregulated while WDR82 was downregulated in LC cell lines, especially in A549 and SPC-A1 cells (Fig. 1f–h). The gene discussed is WDR82; the disease is laryngotracheoesophageal cleft.