In addition, western blotting results revealed decreased levels of EMT-associated cell surface markers and transcription factors in primary tumours derived from sh-NEAT1-transfected U2OS cells, but the levels of epithelial markers were increased (Fig. 7h; p < 0.01 for N-cadherin, p < 0.05 for E-cadherin, Vimentin, and Snail, respectively). This evidence concerns the gene CDH2 and neoplasm.