Finally, the results of western blotting experiments coincided with the qRT-PCR results and further confirmed the functions of NEAT1 and miR-483 in regulating the EMT in osteosarcoma cells (p < 0.01 for E-cadherin, N-cadherin, Snail in OE-NEAT1 group; p < 0.05 for Vimentin in OE-NEAT1 group; p < 0.01 for N-cadherin and Snail in OE-NEAT1 plus miR-483 mimics group; p < 0.05 for E-cadherin and Vimentin in OE-NEAT1 plus miR-483 mimics group). The gene discussed is NEAT1; the disease is osteosarcoma.