For cancer gene screening, scientists applied iExCN analysis tools combined with CRISPR/Cas9 technology to successfully screen and verify dozens of human RMS cancer-related genes such as oncogenes CDCA2, HAS2, SNAI2, WAR, EZH2, SCARA3, ARL4A, CDK6, ETV1, RAD54B, RIPK2, and ZFHX4, and tumor suppressor genes PTEN, ZRSR2, and TJP2. Many of these genes are related to RSM growth and differentiation [79]. Here, RAD54B is linked to cancer.