Figure 4C shows signal transduction pathways affected by significant mutations in colon cancer (Figure 4A). Signaling pathway changes known in TP53 mutated tumors are overrepresented in the list of pathways. A RAS wild type colon cancer patient with HER2+ by CISH and IHC 3+ had 2 pathogenic TP53 mutations affecting both alleles indicating a rare and novel event. This evidence concerns the gene ERBB2 and malignant colon neoplasm.