Using this approach, the composition of extracellular aggregates was investigated by pre-incubating the secretomes from TNF-treated PSEN1 mutant neurons with nanobodies or antibodies to Aβ, α-synuclein and tau, all of which form aggregates in neurodegenerative diseases and are released by neurons in culture (Moore et al., 2015; Evans et al., 2018). This evidence concerns the gene MAPT and neurodegenerative disease.