Cross platform comparison with the Opal Vectra (Perkin Elmer) for a number of markers across similar regions of interest demonstrated concordance for pan-cytokeratin, CD8, and PD-L1.<h4>Conclusion</h4>This study, to our knowledge, represents the first digital spatial analysis of HNSCC tumors. This evidence concerns the gene CD8A and head and neck squamous cell carcinoma.