To investigate the role of KIF3B on Wnt/β-catenin signaling in breast cancer cells, we examined the expression levels of β-catenin, β-catenin (Nucleus), Dvl2, p-GSK-3β, GSK-3β, CyclinD1,C-myc and MMP-7 by using western blot in KIF3B-shRNA MDA-MB-231, MCF-7 cells and KIF3B over-expression MDA-MB-453 cells. The gene discussed is CCND1; the disease is breast carcinoma.