We chose four common CRC cell lines, Caco-2, HCT116, HT-29, and COLO205, and treated those cells with necroptosis inducers TNF + birinapant+zVAD (TBZ) or TNF + birinapant+IDN-6556 (TBI) in the presence or absence of necrostatin-1 (Nec-1s). Here, TNF is linked to colorectal carcinoma.