CASP3 and colorectal carcinoma: Western blot analysis showed that P5 induced caspase-3 and PARP-1 cleavage, but not MLKL phosphorylation in CRC cells and ABIN-1 deficiency enhanced the caspase-3/PARP-1 cleavage, which was not inhibited by Nec-1s (Fig. 2c and Supplementary Fig. S2c).