Furthermore, for TNF-induced necroptosis, sensitization may be due to increases in TNF-induced RIPK1 polyubiquitination by reducing the recruitment of ubiquitin-editing enzyme A20 to the TNFR1 signaling complex and increase in TNF secretion in CRC cells upon pro-necroptosis stimulation. The gene discussed is RIPK1; the disease is colorectal carcinoma.