Next, using a tissue array of primary invasive ductal carcinoma of human breast (n = 133), we conducted immunohistochemistry (IHC) and found that NUPR1 was strongly detected in the nucleus in 30% of the breast tumor tissues, compared with that found by negative staining in adjacent noncancerous breast tissues (Fig. 1D). The gene discussed is NUPR1; the disease is breast neoplasm.