The nuclear-cytoplasmic RNA fractionation and FISH assay were used to analyze the subcellular localization of CCDC183-AS1 in the HCC cells, and the results indicated that CCDC183-AS1 mostly located in the cytoplasm (Fig. 1f-g and Supplementary Fig. 1A-1B). Here, CCDC183 is linked to hepatocellular carcinoma.