Since the MAFs of all SNPs were within the range of 25 to 35%, the relative frequencies (RF) of OB (overweight/obesity) in the mutant allele of all seven MEK6 SNPs were significantly higher than those in the wild allele (Figure 3B) (Supplementary Table S2). This evidence concerns the gene MAP2K6 and obesity due to melanocortin 4 receptor deficiency.