We then selected three genes involved in ovarian function and insulin transport that we found both differentially expressed and/or methylated in PAMH F3 offspring versus controls; namely Sorbs2, Grem1, and Igfbp6. Our qRT-PCR experiments confirmed the RNA-seq data and showed a significant downregulation of Sorbs2 in the ovaries of PCOS animals, while its expression remained unaltered after the SAM treatment (Figure S7A). This evidence concerns the gene GREM1 and polycystic ovary syndrome.