IGFBP6 and polycystic ovary syndrome: We then selected three genes involved in ovarian function and insulin transport that we found both differentially expressed and/or methylated in PAMH F3 offspring versus controls; namely Sorbs2, Grem1, and Igfbp6. Our qRT-PCR experiments confirmed the RNA-seq data and showed a significant downregulation of Sorbs2 in the ovaries of PCOS animals, while its expression remained unaltered after the SAM treatment (Figure S7A).