INS and type 2 diabetes mellitus: In order to investigate whether PAMH mice displayed a T2D-like phenotype, we first applied a tissue-clearing technique, iDISCO+ (Renier et al., 2014), and whole-organ 3D imaging to determine the 3D distribution of insulin-producing β cells and glucagon-producing α cells, across the whole pancreas in 6-month-old CNTR and PAMH F1 female mice (Figure 2E).